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medium b permeabilization buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher medium b permeabilization buffer
    Medium B Permeabilization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/medium+b+permeabilization+buffer/pmc11707799-267-36-40
    Average 90 stars, based on 1 article reviews
    medium b permeabilization buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Death and survival of gut CD4 T cells following HIV-1 infection ex vivo
    Article Snippet: In this case, we used the Database of Immune Cell Expression, Expression quantitative trait loci and Epigenomics (DICE) ( ) to determine the T cell subsets. .. Standard flow cytometry staining protocols were used to stain for viability, cell surface markers (CD3, CD4, CD8, CD45RO, CD120b, GITR, OX40, CD38, HLA-DR, and CCR5), and intracellular expression (GZB, p24, perforin) after Medium A Fixation and Medium B Permeabilization buffer (Life Technologies). ..

    Article Title: Granzyme B + CD4 T cells accumulate in the colon during chronic HIV-1 infection
    Article Snippet: .. Specifically, standard flow cytometry staining protocols were used to determine expression of viable cells (Aqua Zombie Live/Dead Fixable cell dye, Biolegend), cell surface markers CD3 (BV605 CD3, clone UCHT1; Biolegend), CD4 (BUV395 CD4, clone SK2, BD Biosciences), CD8 (PE Dazzle, clone RPA-T8, Biolegend), CCR7 (APC-Cy7 CCR7, clone G043H7, Biolegend) and CD45RO (PE CD45RO, clone UCHL1, Biolegend) and intracellular expression of GZB (Pacific Blue GZB, clone GB11, Biolegend) in Medium A Fixation buffer and Medium B Permeabilization buffer (both Life Technologies, Thermo Fisher Scientific) as previously detailed., , Fluorochrome matched isotype controls were used to establish naïve (CCR7 + CD45RO − ), central memory (CM, CCR7 + CD45RO + ), effector memory (EM, CCR7 − CD45RO + ), and terminally differentiated effector memory (TD, CCR7 − CD45RO − ) CD4 T cells and intracellular GZB expression (mouse IgG1). .. Data was acquired on an LSRII flow cytometer (BD Biosciences) with routine quality control by Cytometer Setup and Tracking feature within the BD FACSDiva software version 6.1.2 (BD Biosciences) performed routinely.

    Staining:

    Article Title: Death and survival of gut CD4 T cells following HIV-1 infection ex vivo
    Article Snippet: In this case, we used the Database of Immune Cell Expression, Expression quantitative trait loci and Epigenomics (DICE) ( ) to determine the T cell subsets. .. Standard flow cytometry staining protocols were used to stain for viability, cell surface markers (CD3, CD4, CD8, CD45RO, CD120b, GITR, OX40, CD38, HLA-DR, and CCR5), and intracellular expression (GZB, p24, perforin) after Medium A Fixation and Medium B Permeabilization buffer (Life Technologies). ..

    Article Title: MAIT cells are functionally impaired in a Mauritian cynomolgus macaque model of SIV and Mtb co-infection
    Article Snippet: .. For intracellular staining, cells were washed twice with FACS buffer and staining with antibodies was performed in Medium B permeabilization buffer (Thermo Fisher Scientific, Cat. No. GAS002S-100) for 20 minutes at room temperature. .. For intranuclear staining, the True Nuclear Transcription Factor Buffer Set (Biolegend; San Diego, CA) was used according to manufacturer’s instructions.

    Article Title: Granzyme B + CD4 T cells accumulate in the colon during chronic HIV-1 infection
    Article Snippet: .. Specifically, standard flow cytometry staining protocols were used to determine expression of viable cells (Aqua Zombie Live/Dead Fixable cell dye, Biolegend), cell surface markers CD3 (BV605 CD3, clone UCHT1; Biolegend), CD4 (BUV395 CD4, clone SK2, BD Biosciences), CD8 (PE Dazzle, clone RPA-T8, Biolegend), CCR7 (APC-Cy7 CCR7, clone G043H7, Biolegend) and CD45RO (PE CD45RO, clone UCHL1, Biolegend) and intracellular expression of GZB (Pacific Blue GZB, clone GB11, Biolegend) in Medium A Fixation buffer and Medium B Permeabilization buffer (both Life Technologies, Thermo Fisher Scientific) as previously detailed., , Fluorochrome matched isotype controls were used to establish naïve (CCR7 + CD45RO − ), central memory (CM, CCR7 + CD45RO + ), effector memory (EM, CCR7 − CD45RO + ), and terminally differentiated effector memory (TD, CCR7 − CD45RO − ) CD4 T cells and intracellular GZB expression (mouse IgG1). .. Data was acquired on an LSRII flow cytometer (BD Biosciences) with routine quality control by Cytometer Setup and Tracking feature within the BD FACSDiva software version 6.1.2 (BD Biosciences) performed routinely.

    Article Title: Spontaneous control of SIV replication does not prevent immune dysregulation and bacterial dissemination in animals co-infected with M. tuberculosis
    Article Snippet: .. For intracellular staining, cells were washed twice with FACS buffer and staining with antibodies was performed in Medium B permeabilization buffer (Thermo Fisher Scientific, Cat. No. GAS002S-100) for 20 minutes at room temperature. .. For intranuclear staining, the True Nuclear Transcription Factor Buffer Set (Biolegend; San Diego, CA) was used according to manufacturer’s instructions.

    Article Title: Spontaneous Control of SIV Replication Does Not Prevent T Cell Dysregulation and Bacterial Dissemination in Animals Co-Infected with M. tuberculosis
    Article Snippet: .. Samples were fixed in 1% paraformaldehyde for a minimum of 20 min. For intracellular staining, cells were washed twice with FACS buffer and staining with antibodies was performed in Medium B permeabilization buffer (Thermo Fisher Scientific, Cat. No. GAS002S-100) for 20 min at room temperature. .. For intranuclear staining, the True Nuclear Transcription Factor Buffer Set (Biolegend; San Diego, CA) was used according to manufacturer’s instructions.

    Expressing:

    Article Title: Death and survival of gut CD4 T cells following HIV-1 infection ex vivo
    Article Snippet: In this case, we used the Database of Immune Cell Expression, Expression quantitative trait loci and Epigenomics (DICE) ( ) to determine the T cell subsets. .. Standard flow cytometry staining protocols were used to stain for viability, cell surface markers (CD3, CD4, CD8, CD45RO, CD120b, GITR, OX40, CD38, HLA-DR, and CCR5), and intracellular expression (GZB, p24, perforin) after Medium A Fixation and Medium B Permeabilization buffer (Life Technologies). ..

    Article Title: Granzyme B + CD4 T cells accumulate in the colon during chronic HIV-1 infection
    Article Snippet: .. Specifically, standard flow cytometry staining protocols were used to determine expression of viable cells (Aqua Zombie Live/Dead Fixable cell dye, Biolegend), cell surface markers CD3 (BV605 CD3, clone UCHT1; Biolegend), CD4 (BUV395 CD4, clone SK2, BD Biosciences), CD8 (PE Dazzle, clone RPA-T8, Biolegend), CCR7 (APC-Cy7 CCR7, clone G043H7, Biolegend) and CD45RO (PE CD45RO, clone UCHL1, Biolegend) and intracellular expression of GZB (Pacific Blue GZB, clone GB11, Biolegend) in Medium A Fixation buffer and Medium B Permeabilization buffer (both Life Technologies, Thermo Fisher Scientific) as previously detailed., , Fluorochrome matched isotype controls were used to establish naïve (CCR7 + CD45RO − ), central memory (CM, CCR7 + CD45RO + ), effector memory (EM, CCR7 − CD45RO + ), and terminally differentiated effector memory (TD, CCR7 − CD45RO − ) CD4 T cells and intracellular GZB expression (mouse IgG1). .. Data was acquired on an LSRII flow cytometer (BD Biosciences) with routine quality control by Cytometer Setup and Tracking feature within the BD FACSDiva software version 6.1.2 (BD Biosciences) performed routinely.

    FACS:

    Article Title: MAIT cells are functionally impaired in a Mauritian cynomolgus macaque model of SIV and Mtb co-infection
    Article Snippet: .. For intracellular staining, cells were washed twice with FACS buffer and staining with antibodies was performed in Medium B permeabilization buffer (Thermo Fisher Scientific, Cat. No. GAS002S-100) for 20 minutes at room temperature. .. For intranuclear staining, the True Nuclear Transcription Factor Buffer Set (Biolegend; San Diego, CA) was used according to manufacturer’s instructions.

    Article Title: Spontaneous control of SIV replication does not prevent immune dysregulation and bacterial dissemination in animals co-infected with M. tuberculosis
    Article Snippet: .. For intracellular staining, cells were washed twice with FACS buffer and staining with antibodies was performed in Medium B permeabilization buffer (Thermo Fisher Scientific, Cat. No. GAS002S-100) for 20 minutes at room temperature. .. For intranuclear staining, the True Nuclear Transcription Factor Buffer Set (Biolegend; San Diego, CA) was used according to manufacturer’s instructions.

    Article Title: Spontaneous Control of SIV Replication Does Not Prevent T Cell Dysregulation and Bacterial Dissemination in Animals Co-Infected with M. tuberculosis
    Article Snippet: .. Samples were fixed in 1% paraformaldehyde for a minimum of 20 min. For intracellular staining, cells were washed twice with FACS buffer and staining with antibodies was performed in Medium B permeabilization buffer (Thermo Fisher Scientific, Cat. No. GAS002S-100) for 20 min at room temperature. .. For intranuclear staining, the True Nuclear Transcription Factor Buffer Set (Biolegend; San Diego, CA) was used according to manufacturer’s instructions.

    Recombinase Polymerase Amplification:

    Article Title: Granzyme B + CD4 T cells accumulate in the colon during chronic HIV-1 infection
    Article Snippet: .. Specifically, standard flow cytometry staining protocols were used to determine expression of viable cells (Aqua Zombie Live/Dead Fixable cell dye, Biolegend), cell surface markers CD3 (BV605 CD3, clone UCHT1; Biolegend), CD4 (BUV395 CD4, clone SK2, BD Biosciences), CD8 (PE Dazzle, clone RPA-T8, Biolegend), CCR7 (APC-Cy7 CCR7, clone G043H7, Biolegend) and CD45RO (PE CD45RO, clone UCHL1, Biolegend) and intracellular expression of GZB (Pacific Blue GZB, clone GB11, Biolegend) in Medium A Fixation buffer and Medium B Permeabilization buffer (both Life Technologies, Thermo Fisher Scientific) as previously detailed., , Fluorochrome matched isotype controls were used to establish naïve (CCR7 + CD45RO − ), central memory (CM, CCR7 + CD45RO + ), effector memory (EM, CCR7 − CD45RO + ), and terminally differentiated effector memory (TD, CCR7 − CD45RO − ) CD4 T cells and intracellular GZB expression (mouse IgG1). .. Data was acquired on an LSRII flow cytometer (BD Biosciences) with routine quality control by Cytometer Setup and Tracking feature within the BD FACSDiva software version 6.1.2 (BD Biosciences) performed routinely.



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